| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.234807 |
| Chromosome: | chromosome_14 |
| Location: | 963396 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre14.g613950 | ABC2 | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CATGAACAGCTACCCCCTCCCCCGCCCCCT |
| Internal bar code: | GTTGTCTCAGTCTCAGTTACGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 176 |
| LEAP-Seq percent confirming: | 82.3529 |
| LEAP-Seq n confirming: | 70 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTGTGGAGTTCTAGCCAGC |
| Suggested primer 2: | TCTTACCAATCTTGGCACCC |