| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.235130 |
| Chromosome: | chromosome 9 |
| Location: | 3490001 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g389615 | MAC1 | psaC mRNA stabilization factor; (1 of 1) PTHR23083//PTHR23083:SF403 - TETRATRICOPEPTIDE REPEAT PROTEIN, TPR // SUBFAMILY NOT NAMED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCTAAGCTCAAGTGCCCAACTGCTTAATC |
| Internal bar code: | GGCGGCACCCGCAAGTGCCTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 601 |
| LEAP-Seq percent confirming: | 99.4143 |
| LEAP-Seq n confirming: | 679 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAATGGCCAAGCAGGATAGA |
| Suggested primer 2: | TGGGACAGTATACGGTGGGT |