| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.235358 |
| Chromosome: | chromosome 6 |
| Location: | 1511987 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g259800 | CWC22 | (1 of 1) K13100 - pre-mRNA-splicing factor CWC22 (CWC22); Cwc22 pre-mRNA splicing factor | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCTCTCCCAACACCCCCACTCCTCACACT |
| Internal bar code: | GCTTGAATCTAGGAAATTAGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 354 |
| LEAP-Seq percent confirming: | 99.4253 |
| LEAP-Seq n confirming: | 1384 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCTTTCCATCAAACCTGCC |
| Suggested primer 2: | ACACACGCATACAGGAGCTG |