| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.235358 |
| Chromosome: | chromosome 6 |
| Location: | 1512397 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g259800 | CWC22 | (1 of 1) K13100 - pre-mRNA-splicing factor CWC22 (CWC22); Cwc22 pre-mRNA splicing factor | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCCAGGAGGGGCGCCGGTGAAGTGCCGAG |
| Internal bar code: | GTGAGCCGTAGAAGACGGAAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 326 |
| LEAP-Seq percent confirming: | 83.7265 |
| LEAP-Seq n confirming: | 1739 |
| LEAP-Seq n nonconfirming: | 338 |
| LEAP-Seq n unique pos: | 33 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACACACGCATACAGGAGCTG |
| Suggested primer 2: | ACCTGGTCCACTACCAGCAG |