Insertion junction: LMJ.RY0402.235430_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.235430
Chromosome:chromosome 6
Location:8468037
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre06.g307500 LCIC,LCIC1 Low-CO2 inducible protein C intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCTTCCCAACGCTCTCGCTTCCAAACCCGC
Internal bar code:GTGTGTCCATGTATTAGGGCCG

Confirmation - LEAP-Seq

LEAP-Seq distance:1096
LEAP-Seq percent confirming:99.211
LEAP-Seq n confirming:9053
LEAP-Seq n nonconfirming:72
LEAP-Seq n unique pos:15

Suggested primers for confirmation by PCR

Suggested primer 1:GCATGCTGTATCAAGGGGTT
Suggested primer 2:TCCAAGAAGCAACACAGCAC