| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.235538 |
| Chromosome: | chromosome 5 |
| Location: | 592086 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g232200 | NDA3,LCI18 | Mitochondrial NADH dehydrogenase; (1 of 1) PTHR22912:SF180 - 3-PHENYLPROPIONATE/CINNAMIC ACID DIOXYGENASE FERREDOXIN--NAD(+) REDUCTASE COMPONENT | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGAGTGACGGACTAGCAGTGATACAAGGAG |
| Internal bar code: | GAGGGTGTTGTTCGACTAGCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 483 |
| LEAP-Seq percent confirming: | 99.0291 |
| LEAP-Seq n confirming: | 918 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGGGTATTCGGTGTGGTGT |
| Suggested primer 2: | GTTCGGCATGTTGACACATC |