| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.235619 |
| Chromosome: | chromosome_1 |
| Location: | 3907631 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre01.g025400 | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GTAACCCACCTGCTTGTAGGGGAATCGCAG |
| Internal bar code: | TCCCGGCTTATACGCGGGCGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 683 |
| LEAP-Seq percent confirming: | 99.33 |
| LEAP-Seq n confirming: | 2817 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 49 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGAAGAAGGAGCTGGACAAC |
| Suggested primer 2: | GTCTGCTCCTACGGCTTCAG |