Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.236192 |
Chromosome: | chromosome 12 |
Location: | 1175819 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre12.g489900 | CYG54 | (1 of 4) PTHR11920//PTHR11920:SF248 - ADENYLATE AND GUANYLATE CYCLASES // SUBFAMILY NOT NAMED; Adenylate/guanylate cyclase | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTAATGATGCATACGTGTCAAGGAAAACAC |
Internal bar code: | CAAGGTTCAGTCGTTACGCTTC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 722 |
LEAP-Seq percent confirming: | 96.5787 |
LEAP-Seq n confirming: | 1976 |
LEAP-Seq n nonconfirming: | 70 |
LEAP-Seq n unique pos: | 43 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | AGTTAGCGAGACACGAGGGA |
Suggested primer 2: | CGTCACAGATGAGTGTCGCT |