| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.236192 |
| Chromosome: | chromosome 12 |
| Location: | 1175825 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g489900 | CYG54 | (1 of 4) PTHR11920//PTHR11920:SF248 - ADENYLATE AND GUANYLATE CYCLASES // SUBFAMILY NOT NAMED; Adenylate/guanylate cyclase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTATGGGAGTGGCCTCTGAACGGACCGGG |
| Internal bar code: | TGGCGCTCAAAGGGTTCGCCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1207 |
| LEAP-Seq percent confirming: | 99.7122 |
| LEAP-Seq n confirming: | 7969 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTTAGCGAGACACGAGGGA |
| Suggested primer 2: | CGTCACAGATGAGTGTCGCT |