| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.237053 |
| Chromosome: | chromosome_12 |
| Location: | 3053059 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre12.g500550 | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | ATGAACCTACACAAAGTGTTTGCAACGGCG |
| Internal bar code: | TCAGGACAGCGGCGCAAAATGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 672 |
| LEAP-Seq percent confirming: | 98.4708 |
| LEAP-Seq n confirming: | 1803 |
| LEAP-Seq n nonconfirming: | 28 |
| LEAP-Seq n unique pos: | 44 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGACCTGATCCTTGACCTGA |
| Suggested primer 2: | GTATGGAGTTGCTGGGGTGT |