| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.237217 |
| Chromosome: | chromosome 15 |
| Location: | 335222 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre15.g635800 | SMC1 | Structural Maintenance of Chromosomes protein; (1 of 1) K06636 - structural maintenance of chromosome 1 (SMC1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAAGATTCTGGGTTTGGATCCTGTCCGCCC |
| Internal bar code: | TATTTCTTTCAGAGTAAAAGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 465 |
| LEAP-Seq percent confirming: | 99.5114 |
| LEAP-Seq n confirming: | 611 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGATCCTGGACAATCCTTT |
| Suggested primer 2: | CCAGGGTGAGTACGTAGGGA |