| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.237342 |
| Chromosome: | chromosome 16 |
| Location: | 1273460 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g651350 | ATG17 | AuTophaGy related; (1 of 1) PTHR13222//PTHR13222:SF1 - CGTHBA PROTEIN -14 GENE PROTEIN // RB1-INDUCIBLE COILED-COIL PROTEIN 1 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCTTAAAGTTAGCGCTTAAAGTTAGCGCT |
| Internal bar code: | CCGTCTGCTCACGGTGATGTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 606 |
| LEAP-Seq percent confirming: | 98.2759 |
| LEAP-Seq n confirming: | 228 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGATTACTCGCCCTCATTG |
| Suggested primer 2: | TGGATCCAAAGGCTAGGATG |