| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.237401 |
| Chromosome: | chromosome 13 |
| Location: | 2469258 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g580000 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCGATGATTGTGCGCTGTATTAGTTATCA |
| Internal bar code: | TATCGGGTGTGTCTTCGCTCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1006 |
| LEAP-Seq percent confirming: | 99.0697 |
| LEAP-Seq n confirming: | 9371 |
| LEAP-Seq n nonconfirming: | 88 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCAGTCTTTCTCAGCCGAA |
| Suggested primer 2: | GTCCACCACGCTCGTTATTT |