Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.237407 |
Chromosome: | chromosome 17 |
Location: | 5302939 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre17.g736250 | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACACATCTATCACTAGCATGGGCCAGCAGG |
Internal bar code: | ACGGGTAGTTTATTTTGCCGGG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 939 |
LEAP-Seq percent confirming: | 99.6909 |
LEAP-Seq n confirming: | 24509 |
LEAP-Seq n nonconfirming: | 76 |
LEAP-Seq n unique pos: | 35 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TGTGTGCTACCTGTCTTGCC |
Suggested primer 2: | GCAGTAGTACCGCCTTGCTC |