| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.237598 |
| Chromosome: | chromosome 5 |
| Location: | 1037041 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g246377 | ADA2 | Adenosine/AMP deaminase; (1 of 1) 3.5.4.2 - Adenine deaminase / Adenine aminase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCTTTTGCATTGGGTTCTACGACCCCCTC |
| Internal bar code: | GGACACGGACGAAAAACAGCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 306 |
| LEAP-Seq percent confirming: | 52.7985 |
| LEAP-Seq n confirming: | 283 |
| LEAP-Seq n nonconfirming: | 253 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGCAACAAGAGCGAGCAGA |
| Suggested primer 2: | TATGTGTGCGTGTGTTGGTG |