| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.237815 |
| Chromosome: | chromosome_13 |
| Location: | 337760 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre13.g564350 | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GAGTTGAGGGATGTGGTGGAGCTGCAGCAG |
| Internal bar code: | TTCGGGTTTTTCGGTCGCCGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 314 |
| LEAP-Seq percent confirming: | 99.6614 |
| LEAP-Seq n confirming: | 5298 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTGGTTTAGTTTGGGCTGG |
| Suggested primer 2: | GCAGCAACTGCTGGACATAA |