| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.237847 |
| Chromosome: | chromosome_14 |
| Location: | 964071 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre14.g613950 | ABC2 | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TCTTGGCACCCCCCCTCACCGCCCTCCTCC |
| Internal bar code: | AACATATACTAGTGTCAGAACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 287 |
| LEAP-Seq percent confirming: | 98.6515 |
| LEAP-Seq n confirming: | 951 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGACTTAGTTCAGAAGGCGG |
| Suggested primer 2: | GGAGGGAGGTGGGTTACATT |