| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.237909 |
| Chromosome: | chromosome 9 |
| Location: | 2878247 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g388400 | RAT1 | (1 of 1) PF07798 - Protein of unknown function (DUF1640) (DUF1640); Chloroplast tscA maturation factor | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGGGTCCAGCGGAGGTATCATACCGTACA |
| Internal bar code: | GGAGGGGGAAGTGAGGTGAAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 521 |
| LEAP-Seq percent confirming: | 99.4565 |
| LEAP-Seq n confirming: | 183 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTGCGGTTGTATCATCCCT |
| Suggested primer 2: | AGTCGGTCATCACCATCCTC |