| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.237913 |
| Chromosome: | chromosome 1 |
| Location: | 1831359 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g009900 | CDJ3 | Chloroplast DnaJ-like protein 3; (1 of 2) IPR001080//IPR001623//IPR017896 - 3Fe-4S ferredoxin // DnaJ domain // 4Fe-4S ferredoxin-type, iron-sulphur binding domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGATGCATACTTCGCTCTCTCGCACCTCT |
| Internal bar code: | GCTGATGAGATACGTGTCCTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 328 |
| LEAP-Seq percent confirming: | 98.0066 |
| LEAP-Seq n confirming: | 295 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGCGTCCTACGTTTGATTA |
| Suggested primer 2: | CCCGAAAGTGTTCCTTTCAA |