| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.238007 |
| Chromosome: | chromosome 1 |
| Location: | 4119752 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g027150 | CPLD46,HEL5 | Conserved in the plant lineage and diatoms; (1 of 2) PTHR24031//PTHR24031:SF249 - RNA HELICASE // ATP-DEPENDENT DNA HELICASE RECG | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGCGTCCGGGAGCAGCAGCTCCACGTCGG |
| Internal bar code: | TGTTGTGTTAGGATCTAGTAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1230 |
| LEAP-Seq percent confirming: | 97.9221 |
| LEAP-Seq n confirming: | 10556 |
| LEAP-Seq n nonconfirming: | 224 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGCGTTGGCCTTGAGCTAT |
| Suggested primer 2: | TCAATGGTGTTGGTGGTTGT |