| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.238034 |
| Chromosome: | chromosome 14 |
| Location: | 962985 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g613950 | ABCA2,ABC2 | (1 of 2) 3.6.3.32 - Quaternary-amine-transporting ATPase; ABC lipid exporter 2 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATGTGGATGTCGACACTGATGAGAGGATG |
| Internal bar code: | AGGCGTAACAGGTTGGGAGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 695 |
| LEAP-Seq percent confirming: | 98.7503 |
| LEAP-Seq n confirming: | 4425 |
| LEAP-Seq n nonconfirming: | 56 |
| LEAP-Seq n unique pos: | 97 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGGGGTAGCTGTTCATGTG |
| Suggested primer 2: | CCTGTGGAGTTCTAGCCAGC |