| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.238538 |
| Chromosome: | chromosome 6 |
| Location: | 2749275 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g270850 | SRH12 | (1 of 1) K10841 - DNA excision repair protein ERCC-6 (ERCC6, CSB, RAD26); SNF2-related DNA/RNA helicase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTAACACCTGCTGCGCACTCCTACCTGCTT |
| Internal bar code: | AGCCGTTGGCATGAATACCTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 330 |
| LEAP-Seq percent confirming: | 97.9774 |
| LEAP-Seq n confirming: | 4602 |
| LEAP-Seq n nonconfirming: | 95 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGGTGGTGACCTTTTTGGA |
| Suggested primer 2: | GACGTTAGGGATGTGAGGGA |