| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.238541 |
| Chromosome: | chromosome 12 |
| Location: | 3882221 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g515700 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TATTTAATGTTGGTGTCTGGGAATGCGCTG |
| Internal bar code: | GTGCTGCTCTACACCGTGGTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 297 |
| LEAP-Seq percent confirming: | 99.7297 |
| LEAP-Seq n confirming: | 369 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGTAGCAAGAAGGGACAAG |
| Suggested primer 2: | AGAGCTTTGCATGGTTGCTT |