| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.239464 |
| Chromosome: | chromosome_3 |
| Location: | 1951532 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre03.g155650 | CLC1 | Clathrin light chain | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TTCTGGGGGCAAGTTCTCAACACGACGGGA |
| Internal bar code: | TGCTGACGACCGGATAATAACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 491 |
| LEAP-Seq percent confirming: | 82.963 |
| LEAP-Seq n confirming: | 112 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCCTAAAGTTCAAACTCGC |
| Suggested primer 2: | AGTTTGAGGAGAAGCACCGA |