| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.239581 |
| Chromosome: | chromosome 5 |
| Location: | 1941105 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g241634 | HAV2 | Histone H2A variant | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGGTGAAGGAGAATGGCCAGCGGCGAGGC |
| Internal bar code: | GGTCACACCAGCTCGGGTTGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 548 |
| LEAP-Seq percent confirming: | 97.0976 |
| LEAP-Seq n confirming: | 736 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACAACACGGACTTGTTCCC |
| Suggested primer 2: | ACAGTGAACGGGTGATGACA |