| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.239673 |
| Chromosome: | chromosome 3 |
| Location: | 4919765 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g179800 | LCI24 | Low-CO2-inducible membrane protein; (1 of 5) PF07466 - Protein of unknown function (DUF1517) (DUF1517) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATGCGACTGTTTGAACTTGGCACCCGTTG |
| Internal bar code: | CAAGTATTGGTTGCAACAAGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 565 |
| LEAP-Seq percent confirming: | 53.8462 |
| LEAP-Seq n confirming: | 7 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACACTCCTCTCGCATTGAA |
| Suggested primer 2: | TGCAAATGGCATCAAATGTT |