| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.239731 |
| Chromosome: | chromosome 3 |
| Location: | 2300288 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g158200 | HDA2 | (1 of 1) K11418 - histone deacetylase 11 (HDAC11); RPD3/HDA1 type histone deacetylase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCATTGCCGTGTCTCGGGCGTCGCGCCGG |
| Internal bar code: | GAGCCACTCGGGAATTGGGTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 544 |
| LEAP-Seq percent confirming: | 84.2336 |
| LEAP-Seq n confirming: | 577 |
| LEAP-Seq n nonconfirming: | 108 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTGATAATTGCGACGTGGT |
| Suggested primer 2: | ATTGGGCTGGCATCTACAAC |