| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.239820 |
| Chromosome: | chromosome 13 |
| Location: | 1247631 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g570500 | (1 of 2) PF13374//PF13424 - Tetratricopeptide repeat (TPR_10) // Tetratricopeptide repeat (TPR_12) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGGGAAAGGCCCACACCATGCAGGGGCGG |
| Internal bar code: | TCAGTTCTCGAACGCCTACTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 534 |
| LEAP-Seq percent confirming: | 98.6111 |
| LEAP-Seq n confirming: | 1846 |
| LEAP-Seq n nonconfirming: | 26 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGAAGGGTGTGAAGGTGTG |
| Suggested primer 2: | AAGAGCATGATCCATGAGGG |