| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.239887 |
| Chromosome: | chromosome 1 |
| Location: | 6799484 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g049000 | PTD1,RAF2,CGL31 | (1 of 1) PTHR12599//PTHR12599:SF8 - PTERIN-4-ALPHA-CARBINOLAMINE DEHYDRATASE // SUBFAMILY NOT NAMED; RuBisCO assembly chaperone, chloroplastic | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCAAATGCATCATACACGTGCTATCACGT |
| Internal bar code: | TTTGCCGTACGTGGTGTTGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 241 |
| LEAP-Seq percent confirming: | 99.6241 |
| LEAP-Seq n confirming: | 1060 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTACTTCATCGCTCGAACA |
| Suggested primer 2: | GAAGCGTGTCAGAAGAAGGG |