Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.239921 |
Chromosome: | chromosome_6 |
Location: | 2650571 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre06.g269950 | EZY21,CDC48,NSG14 | Protein involved in ubiquitin-dependent degradation of ER-bound | sense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | ACTTCATCGAACTCTCTCACGTGTGGCGTG |
Internal bar code: | TGCTCATCTCTGGGTACGCGCC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 582 |
LEAP-Seq percent confirming: | 99.1744 |
LEAP-Seq n confirming: | 961 |
LEAP-Seq n nonconfirming: | 8 |
LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TGTACAACTAAAGCGCCACG |
Suggested primer 2: | AAGTGGTTGGAGGTGTGGAG |