| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.240072 |
| Chromosome: | chromosome 3 |
| Location: | 6097504 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g191600 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACGTGTGATGCGGGAGGACCTGGTGCGTG |
| Internal bar code: | CTCAGCGCAAGGAGTTGCGCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 266 |
| LEAP-Seq percent confirming: | 98.1211 |
| LEAP-Seq n confirming: | 470 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCGCCCTCATCGTCTAATG |
| Suggested primer 2: | CCCTGTTTGACAACTCCGTT |