Insertion junction: LMJ.RY0402.240156_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.240156
Chromosome:chromosome 11
Location:2164530
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre11.g469600 HDA9 (1 of 1) IPR000286//IPR002110//IPR020683//IPR023801 - Histone deacetylase superfamily // Ankyrin repeat // Ankyrin repeat-containing domain // Histone deacetylase domain; RPD3/HDA1 type histone deacetylase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACCCTCCTCATCCTCCGAATCCTCCTCCTC
Internal bar code:ATAGTAGGCAACGCTGCAGCGA

Confirmation - LEAP-Seq

LEAP-Seq distance:529
LEAP-Seq percent confirming:98.4375
LEAP-Seq n confirming:63
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:CGTCGTCTTCATCGTCTTCA
Suggested primer 2:TCACGAGTGTACCCCCTTTC