| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.240206 |
| Chromosome: | chromosome 9 |
| Location: | 5832750 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g402219 | LCI3 | Low-CO2-inducible protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAACCCGCAACGCGTGCGCGCCAGCGTTGA |
| Internal bar code: | GCGGTGGAATCGCTCTGCACCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 602 |
| LEAP-Seq percent confirming: | 99.2754 |
| LEAP-Seq n confirming: | 137 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGATTGCGGTCTGATCATCT |
| Suggested primer 2: | TTTCTCCTGATCCATCCCTG |