Insertion junction: LMJ.RY0402.240699_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.240699
Chromosome:chromosome_7
Location:4073975
Confidence (%):95
Locus systematic id Locus common name Defline Orientation Feature
Cre07.g340450 antisense intron

Insertion site details

Flanking sequence (orientation from cassette outwards):TTTTGCAACGACTTTATTGGCGGGGGGGGG
Internal bar code:ATTCCTACACGCAACCACCCTA

Confirmation - LEAP-Seq

LEAP-Seq distance:111
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:10
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:CCGATGTTGTTCTCCAGGAT
Suggested primer 2:CCTAGACGTTTTCAGCTGCC