| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.240911 |
| Chromosome: | chromosome 12 |
| Location: | 8334969 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g549550 | TUB2,RSP19 | (1 of 2) K07375 - tubulin beta (TUBB); beta-2 tubulin | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGCGGGCAGGTTGCTAGCGCTGATGCTAC |
| Internal bar code: | ATTACGCTCACGCAAGGGACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 114 |
| LEAP-Seq percent confirming: | 97.9167 |
| LEAP-Seq n confirming: | 47 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGAGTGGCAGACCTGGAAG |
| Suggested primer 2: | AGCGAGCTACCAAAGCCATA |