Insertion junction: LMJ.RY0402.241725_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.241725
Chromosome:chromosome 6
Location:5430005
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre06.g284200 LHCBM9 (1 of 6) K08912 - light-harvesting complex II chlorophyll a/b binding protein 1 (LHCB1); Light-harvesting Chlorophyll a/b binding protein of LHCII 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTTCTGGATGTACCACGGATAGGGCCCACG
Internal bar code:GCTCGTGTCCGAGGGTGGGCTC

Confirmation - LEAP-Seq

LEAP-Seq distance:579
LEAP-Seq percent confirming:98.1116
LEAP-Seq n confirming:1143
LEAP-Seq n nonconfirming:22
LEAP-Seq n unique pos:25

Suggested primers for confirmation by PCR

Suggested primer 1:GCAATAGGGGTTCTATCGCA
Suggested primer 2:GCGGTGTAGTCTAGCCAAGC