| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.241782 |
| Chromosome: | chromosome 16 |
| Location: | 1928634 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g656150 | (1 of 1) PTHR23504:SF1 - PROTEIN T25D3.4 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCGTGGCCATACGACACGCATATGGCGTC |
| Internal bar code: | ATGAGGCGCTCGGCAAGATCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 315 |
| LEAP-Seq percent confirming: | 82.8571 |
| LEAP-Seq n confirming: | 29 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGCACCTTAGCAGCAAAAT |
| Suggested primer 2: | CACGAAGTTTGTCCAGCTCA |