Insertion junction: LMJ.RY0402.241959_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.241959
Chromosome:chromosome 10
Location:3197357
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre10.g442600 UAPA2,XUV5 Xanthine/uracil/vitamin C permease-like; (1 of 4) PTHR11119//PTHR11119:SF36 - XANTHINE-URACIL / VITAMIN C PERMEASE FAMILY MEMBER // SUBFAMILY NOT NAMED 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCGCTAGGTGCCCTACTGAAGAGGCGAAG
Internal bar code:CCAGACGGATCGAGGAGGTCAT

Confirmation - LEAP-Seq

LEAP-Seq distance:79
LEAP-Seq percent confirming:99.1531
LEAP-Seq n confirming:1405
LEAP-Seq n nonconfirming:12
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:GTAACCTTTCGGACCGTTGA
Suggested primer 2:GGGAACACTTGTCGTGTGTG