| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.242559 |
| Chromosome: | chromosome 1 |
| Location: | 5586905 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g039650 | PDF1C | (1 of 3) 3.5.1.88 - Peptide deformylase / Polypeptide deformylase; Peptide deformylase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTTGTAAGTCCTTTGTCCCAACTACTACG |
| Internal bar code: | CAGTATGCACTGGAGTAGAGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 455 |
| LEAP-Seq percent confirming: | 99.3103 |
| LEAP-Seq n confirming: | 144 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCTACAAGTCACCCGGTTC |
| Suggested primer 2: | GTTGACCAGGATGGACTCGT |