| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.242574 |
| Chromosome: | chromosome 2 |
| Location: | 78621 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g073700 | UROD3,UPD3 | (1 of 1) PTHR21091//PTHR21091:SF100 - METHYLTETRAHYDROFOLATE:HOMOCYSTEINE METHYLTRANSFERASE RELATED // SUBFAMILY NOT NAMED; Uroporphyrinogen-III decarboxylase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTTGACATGGTCAAGGGCAAGGGCCCCGT |
| Internal bar code: | CATTGGACCAAGTGCTGGGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 456 |
| LEAP-Seq percent confirming: | 98.0392 |
| LEAP-Seq n confirming: | 100 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAGCGAGAACTCCGTAAAC |
| Suggested primer 2: | TGATTGCGCTCTTTTGTGAC |