| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.242850 |
| Chromosome: | chromosome 7 |
| Location: | 4648126 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g344400 | (1 of 3) 1.1.1.95 - Phosphoglycerate dehydrogenase / Phosphoglyceric acid dehydrogenase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCACGCTTGTCACACCGTGGTAAACAGTA |
| Internal bar code: | GGTAAGGGCGGTGGGTCGTTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 67 |
| LEAP-Seq percent confirming: | 98.7342 |
| LEAP-Seq n confirming: | 156 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGATCGTTGACAAGCGACA |
| Suggested primer 2: | TTGAGCAGCAGATCAACACC |