Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.243070 |
Chromosome: | chromosome_12 |
Location: | 3048947 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre12.g500550 | sense | 5'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CAGGACACGAAAGGCCAAGAAGGGCACTTC |
Internal bar code: | CGAAACTATCGCATCCGAGCTC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 405 |
LEAP-Seq percent confirming: | 99.0132 |
LEAP-Seq n confirming: | 301 |
LEAP-Seq n nonconfirming: | 3 |
LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GTAAGGGTTACGTACCGCGA |
Suggested primer 2: | GCTGCTGCTGGTAATGGAAT |