| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.243435 |
| Chromosome: | chromosome 9 |
| Location: | 2054777 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g394150 | RAA1,OPR40 | psaA mRNA trans-splicing factor; (1 of 3) PF06743 - FAST kinase-like protein, subdomain 1 (FAST_1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCAAACCATGTAGCCTCCTTAGTGCAATAG |
| Internal bar code: | ATTGCGGGATCCGATTTTTATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 84 |
| LEAP-Seq percent confirming: | 85.7143 |
| LEAP-Seq n confirming: | 6 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACCCCTTTACGTTGTGCTG |
| Suggested primer 2: | CATACTGAGGTCCGAGCCAT |