Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.243919 |
Chromosome: | chromosome_16 |
Location: | 2275330 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre16.g659050 | sense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TTGGCGTCCAGGTCTCCCACCACCAGCACC |
Internal bar code: | CGGACTGATTTTGTGCCACGCA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 209 |
LEAP-Seq percent confirming: | 99.8002 |
LEAP-Seq n confirming: | 4495 |
LEAP-Seq n nonconfirming: | 9 |
LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GCTTCTTCCAGACAAGCCAT |
Suggested primer 2: | ATGCATGCACCACACTCAAT |