| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.244952 |
| Chromosome: | chromosome 10 |
| Location: | 2401568 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g435400 | (1 of 1) PF03398 - Regulator of Vps4 activity in the MVB pathway (Ist1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCTGGTCGGGTCGGGCGTCGTCAGGAGAG |
| Internal bar code: | TTAGTTTTCCAGCGCTTATTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 331 |
| LEAP-Seq percent confirming: | 67.4927 |
| LEAP-Seq n confirming: | 9696 |
| LEAP-Seq n nonconfirming: | 4670 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCAGCAGCATCATATACGCC |
| Suggested primer 2: | AGCAGCACTGGAGATGGAGT |