| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.245235 |
| Chromosome: | chromosome 3 |
| Location: | 5082947 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g181250 | SNE18,CGLD13 | (1 of 10) PF13460 - NAD(P)H-binding (NAD_binding_10); conserved protein related to nucleoside diphosphate sugar epimerase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTTCTACAATGACCGTGAAACCAAGGAGC |
| Internal bar code: | AATCCTGCGTCCAGTGAATTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1001 |
| LEAP-Seq percent confirming: | 99.216 |
| LEAP-Seq n confirming: | 7973 |
| LEAP-Seq n nonconfirming: | 63 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGACTCTCGACTTCTCCGAC |
| Suggested primer 2: | ATGCCCTGCCTACGTGTTAC |