Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.245687 |
Chromosome: | chromosome_16 |
Location: | 1159923 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre16.g650450 | FAP219 | Flagellar Associated Protein | sense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | AACCGCGCCGCGGCTTGCGGGTCCATGCTA |
Internal bar code: | AAGTCATGCATTGCGACGGCAC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 263 |
LEAP-Seq percent confirming: | 98.1145 |
LEAP-Seq n confirming: | 2758 |
LEAP-Seq n nonconfirming: | 53 |
LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CACTCAGCCCACAACTCAGA |
Suggested primer 2: | AAGTTGGTGGTGTAGCCCTG |