Insertion junction: LMJ.RY0402.245980_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.245980
Chromosome:chromosome 1
Location:7324915
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre01.g052750 (1 of 1) PF06011//PF13418 - Transient receptor potential (TRP) ion channel (TRP) // Galactose oxidase, central domain (Kelch_4); Transient receptor potential ion channel intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGCATATCTGCCCACTTCCCGCACACCTC
Internal bar code:GTTTCGCGCCACGCCGGATTG

Confirmation - LEAP-Seq

LEAP-Seq distance:84
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:51
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:GATTGTCTGACACACACCCG
Suggested primer 2:GGAACAATCCATCCGAGAGA