| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.246040 |
| Chromosome: | chromosome_6 |
| Location: | 5347049 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre06.g283500 | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | AGTCCAGCCCTGGCGATTGCGCCGTGCCGC |
| Internal bar code: | GTAGGATCCGGTGGTTAGGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 556 |
| LEAP-Seq percent confirming: | 99.2844 |
| LEAP-Seq n confirming: | 555 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTGCAAACCCTTGACAACC |
| Suggested primer 2: | GCATTGCGGGTTTTAGACAT |