Insertion junction: LMJ.RY0402.246123_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.246123
Chromosome:chromosome 6
Location:2751686
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre06.g270850 SRH12 (1 of 1) K10841 - DNA excision repair protein ERCC-6 (ERCC6, CSB, RAD26); SNF2-related DNA/RNA helicase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTTCCTCACAAACAAGGTGGGGTTGTAGAT
Internal bar code:AAGCACTCGGAATTCGCCAACC

Confirmation - LEAP-Seq

LEAP-Seq distance:463
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:33
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:CACAACCAAGGTGGGAGTCT
Suggested primer 2:CCCTTCTTCCGCTTCTTACC